Next- Generation DNA sequencing Review Article By SITA DAHAL postpone of contents Table of Contents2 Abstract:3 Introduction:3 Different sequencing Technologies and Data accumulation:4 SEQUENCING BY entailment:4 SEQUENCING BY HYBRIDIZATION AND LIGATION:4 SINGLE pinch SEQUENCING:5 Assembly of Huge Volume of Data from NGS:7 Applications:7 Discussion:7 Conclusion:9 manoeuver of reference list:10 Abstract: The topic, DNA sequencing has been at hype from the remnant two decades. This review tries to unravel the miscellaneous facet of this engineering and its wide range of applications not moreover in biomedical research but also in another(prenominal) aras. at that place have been rapid technological advancements fashioning most of the broadcast obsolete. At present, second generation sequencers argon being replaced by third generation sequencers having tenacious orders. The richness of bioinformatics and various other questions like where will such gigantic amount of info lead us atomic number 18 dealt. We psychoanalyse by this lease that completion of human genome sequencing was not the remainder but bonnie a starting point to change medicine.
Genome sequencing has helped to develop new-sprung(prenominal) vaccines and the drastic reduction in cost of sequencing platform has make it possible to be used as clinical symptomatic instrument in near future. Introduction: In 1977, Fredrick Sanger demonstrable the first sequencing technique known as ambit margin method. This procedure utilizes dideoxynucleoside triphosphates (ddTTP) which are labele d with radioactive phosphate. The incorpora! tion of ddTTP (lacks 3 OH group) terminates the template directed synthesis of the complementary strand. The strands are denatured, size fractioned on four different lanes and the base sequences are read by auto radiography (Metzker, M.L., 2010). Using this procedure, bacteriophage ?X 174 was first sequenced genome. It is a less error-prone method and produces long reads of approximately 700 bases but endorses various drawbacks like...If you want to remove a full essay, order it on our website: OrderCustomPaper.com
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